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Título:
Biofunctionalization of REDV elastin-like recombinamers improves endothelialization on CoCr alloy surfaces for cardiovascular applications
Fecha de publicación:
2015
Forma de obra:
Libros de texto
Producción:
Colloids and Surfaces B: Biointerfaces; Vol. 127, 2015
Idioma:
inglés
Nota de edición:
Digitalización realizada por la Biblioteca Virtual del Banco de la República (Colombia)
Autor:
Castellanos Arboleda, María Isabel; Gil, Francisco Javier; Grau, Ana; Manero, José María; Pergueroles, Marta; Rodríguez Cabello, José Carlos; Zenses, Anne Sophie
Materia:
Ciencias naturales y matemáticas; Ciencias naturales y matemáticas / Ciencias de la vida Biología; Tecnología; Tecnología / Ciencias médicas Medicina
Nota general:
© Derechos reservados del autor
Nota general:
Colfuturo
Nota general:
Biomimetic materials; CoCr alloy; Elastin-like recombinamers; Endothelialization; Surface functionalization
Nota general:
To improve cardiovascular implant success, metal-based stents are designated to modulate endothelial cells adhesion and migration in order to prevent restenosis and late thrombosis diseases. Biomimetic coatings with extra-cellular matrix adhesive biomolecules onto stents surfaces are a strategy to recover a healthy endothelium.
However, the appropriate bioactive sequences to selective promote growth of endothelium and the biomolecules surface immobilization strategy remains to be elucidated. In this study, biofunctionalization of cobalt chromium, CoCr, alloy surfaces with elastin-like recombinamers, ELR, genetically modified with an REDV sequence, was performed to enhance metal surfaces endothelialization.
Moreover, physical adsorption and covalent bonding were used as biomolecules binding strategies onto CoCr alloy. Surfaces were activated with plasma and etched with sodium hydroxide previous to silanization with 3-chloropropyltriethoxysilane and functionalized with the ELR. CoCr alloy surfaces were successfully biofunctionalized and the use of an ELR with an REDV sequence, allows conferring bioactivity to the biomaterials surface, demonstrating a higher cell adhesion and spreading of HUVEC cells on the different CoCr surfaces.
This effect is emphasized as increases the amount of immobilized biomolecules and directly related to the immobilization technique, covalent bonding, and the increase of surface charge electronegativity. Our strategy of REDV elastin-like recombinamers immobilization onto CoCr alloy surfaces via covalent bonding through organosilanes provides a bioactive surface that promotes endothelial cell adhesion and spreading.
Enlace permanente:
http://www.cervantesvirtual.com/obra/biofunctionalization-of-redv-elastin-like-recombinamers-improves-endothelialization-on-cocr-alloy-surfaces-for-cardiovascular-applications-867051

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